Abstract The traditional educational practice for college students confined to a short-period training was limited in time and activities,and did not suit the cultivation of middle school teachers under the new curriculum concept. Therefore, based on the curriculum reform and teaching practice, this article put forward the cooperative-operating mode of educational practice bases for students major in chemistry, in which the education practice was normalized, the pre-service training was standardized, the activities in the practice bases were systematized, the educational resources could be shared and the summing-up of the practice was sustained. Through this cooperative-operating mode, research level and coaching ability of teachers in both sides were enhanced, and trainees also gained comprehensive development and promotion in teaching.