Abstract This paper investigated 103 chemistry teachers of senior high school of Guangdong province with the help of Questionnaire of teaching concept of the chemistry teachers, then according to the results of investigation, 7 chemistry teachers with different teaching concept were chosen as samples for classroom observation. The questionnaire showed that chemistry teachers from different levels of high school had different orientation of teaching concept, teachers from key schools tended to adopt conception of developmental modes of teaching while teachers from ordinary schools adopted indoctrinating conception of teaching. The classroom observation indicated that chemistry teachers' teaching concept influenced their teaching method, thus we could determine chemistry teachers' teaching concept using classroom observation. Teachers with the same teaching concept from different schools showed no obvious difference in classroom observation.