Abstract Under different mixing condition, through changing reactants' concentration and the ratio of amount of substance, this paper summarized the reaction rules between copper sulfate and sodium hydroxide:the rule of increase and decrease of ions in products, the correspondence rules of the corresponding ions, the change rules of product color. Using these rules, this paper forecasted and prepared yellowish green copper sulfate basic, analyzed and summarized the transformation relationship of products. The reaction rule of copper sulfate and sodium hydroxide was extended to the reaction rule of copper ions and hydroxide ion. These rules could be used as references for methods and theories in the related chemical production research.