Abstract This paper used SPSS 25.0 statistical software to carry out orthogonal experimental design for the “blue bottle” experiment, and obtained the best experimental conditions through statistical analysis. The redox sensor (ORP) was used to analyze the electrode potential change curve of the “blue bottle” oscillation system, and three sets of comparative experiments were designed for the production and disappearance of blue to clarify the reaction principle, which was intended to guide students to understand deeply the nature of the micro-reaction behind the interesting experimental phenomena.
