Design and Practice of Comprehensive Experiment for Separation and Determination of Sulfonamide Residues by Capillary Zone Electrophoresis
BAI Xin-Wei1, YANG Zheng-Long2, LEI Yi-Zhu1, WANG Ke-Liang1, HE Chao1, AN You-Xiao1, HU Zhu-Nan3**
1. School of Chemical Materials, Liupanshui Normal University, Liupanshui 553004, China;
2. School of Chemistry and Materials Science, Ludong University, Yantai 264025, China;
3. School of Educational Science, Liupanshui Normal University,Liupanshui 553004, China
Abstract Combined with scientific research projects, a comprehensive experiment of instrumental analysis suitable for undergraduate teaching was designed by selecting actual samples familiar to students: The comprehensive experimental design and practice of separation and determination of sulfonamide residues by capillary zone electrophoresis. The experimental teaching process of the complete separation and analysis of sulfonamide residues in soil samples and tea samples is shown in detail: Firstly, the students utilize the knowledge of organic chemistry to derivatize the sulfonamide standard and pretreat the actual sample, and then use the knowledge of instrumental analytical chemistry to conduct group discussions to explore the optimal conditions for the separation and analysis of sulfonamide residues by capillary electrophoresis in terms of buffer concentration and pH, and independently design the migration time, peak area repeatability experiments and standard addition recovery experiments to evaluate the repeatability and precision of the experiments, and integrate them into the ideology and politics of food and drug safety courses in real time according to the experiments. Based on this, students are trained to use comprehensive knowledge to analyze and resolve practical problems, the sense ofteam division of labor and the rigorous attitude towards experimental science are cultivated,the barriers between scientific research and teaching are broken, and the leading role of scientific research on experimental teaching is realized.
BAI Xin-Wei, YANG Zheng-Long, LEI Yi-Zhu, WANG Ke-Liang, HE Chao, AN You-Xiao, HU Zhu-Nan. Design and Practice of Comprehensive Experiment for Separation and Determination of Sulfonamide Residues by Capillary Zone Electrophoresis[J]. Chinese Journal of Chemical Education, 2022, 43(18): 92-97.
