Abstract For eluent used in experiment of separating mixed dyes by thin layer chromatography with high toxicity and pollution problems, this paper improved the experiment by adjusting the compositions, proportion and volume of eluent. The final selection of mixed solvents of ethyl acetate and petroleum ether (1:14) was used as an alternative to separate mixed dyes. The experiment results showed that the separation effect of the eluent was obvious, the spots were clear, and the eluent had low toxicity, which conforming to the requirements of the modern green chemistry experiment.
LI Yu-Chen, SHUI Yan-Fang, ZHOU Shao-Jie, ZHU Qi, LIAO Jian-Wei, JIANG Yin-Yan. Improved Experiment of Separating Mixed Dyes by Thin Layer Chromatography[J]. Chinese Journal of Chemical Education, 2016, 37(16): 31-33.