Applications and Case Studies of Tandem MS (MS/MS) in Protein Science
WANG Zhi-Peng1,2, WANG Xin3, LIU Wei1
1. School of Life Science, Tonghua Normal University, Tonghua 134002, China;
2. Department of Chemistry, Texas A&M University, College Station, Texas, 77840, USA;
3. Department of Plant Pathology and Microbiology, Texas A&M University, College Station, Texas, 77843, USA
Abstract Tandem mass spectrometry (MS/MS) is a fast-developing analytical method with broad applications in chemistry and other interdisciplinary researches. Further peak identification is generally needed after computer-assisted spectrum analysis, which is not emphasized or even omitted in chemistry teaching. This paper started with the principles of MS/MS analysis, followed by the systematic summarization of the possible peptide fragmentation and structural characterization in the secondary MS. In addition, this paper introduced spectra calculation methods to illustrate the peptide identification process based on several model proteins. It is our sincere hope that it can provide helpful insights into the protein science education and research.
WANG Zhi-Peng, WANG Xin, LIU Wei. Applications and Case Studies of Tandem MS (MS/MS) in Protein Science[J]. Chinese Journal of Chemical Education, 2017, 38(12): 1-6.
