Tandem mass spectrometry (MS/MS) is a fast-developing analytical method with broad applications in chemistry and other interdisciplinary researches. Further peak identification is generally needed after computer-assisted spectrum analysis, which is not emphasized or even omitted in chemistry teaching. This paper started with the principles of MS/MS analysis, followed by the systematic summarization of the possible peptide fragmentation and structural characterization in the secondary MS. In addition, this paper introduced spectra calculation methods to illustrate the peptide identification process based on several model proteins. It is our sincere hope that it can provide helpful insights into the protein science education and research.

In the case of the integration of scientific research practice, this paper proposed a systematically innovative teaching design of basic concept of coordination compounds for the needs of flipped classroom teaching, which highlighted the fusion of the internet and inorganic chemistry teaching.It was order to enable students to grasp the basic concept of complex and reserve the latest knowledge of the complex for training scientific research and practical ability.Questionnaire results showed that the experimental class students maintained high enthusiasm for the completion of learning tasks, and its comprehensive teaching quality was significantly better than the traditional class. Teaching reform achieved the desired results.

The experiment of determination of caffeine in soft drink by HPLC was reformed and expanded in this study. Procedures were added to remove pigments and carbohydrates which greatly influenced the HPLC analysis, and the mobile phase was modified by using formic acid instead of potassium dihydrogen phosphate buffer. The operability and comprehensiveness of the experiment were enhanced correspondingly, and the result accuracy was also greatly improved. Students got a better cognition of the HPLC method in food analysis. Meanwhile their comprehensive problem solving ability was also enhanced.

Experiments are extremely important in chemistry, whereas the experiment teaching in chemical elective courses is still ineffective. This paper described the introduction of inquiry experiments into the elective couse of separation technique, and illustrated the experiment design and the advantages taking the experiment of the preparation of Schiff base and the identification of fluoride ion by naked eyes as an example. The results showed that the attempt was in favor of improving the comprehensive quality and developing scientific interests of the students who have chosen the course. Moreover, some suggestions were listed based on the improvement of the attempt in future.

In order to solve the problems of traditional teaching of college chemistry experiment, this paper tried to introduce the blended teaching mode based on SPOC. According to the properties of chemical experiment and combing with online and offline teaching, design of blended teaching mode of college chemistry experiment was discussed from three aspects including teaching resources, instructional design and instructional evaluation.

This paper introduced the innovative training program of undergraduate students in Tarim University. A series of modified chitosan anion exchange membrane were prepared with chitosan (CS), 2,3-epoxypropyl trimethyl ammonium chloride and glutaraldehyde under microwave conditions, and properties of products were characterized by IR and TG. The implementation of the project allowed undergraduate students to make full use of what they have learned, and cultivate their competency of scientific research, and played a positive role in training innovative talents.

In this paper, three key factors to the yield of basic copper carbonate preparation including material radio, temperature and reaction time were studied. The composition of prepared basic copper carbonate was also researched in detail with infrared detection, orthogonal experiment and single factor experiment. The results indicated that in the reaction, material radio, temperature and reaction time acted as a key factor for the yield, reaction rate and products composition, separately. Taken together, the study further exhibited that with a volume ratio of 1:1.2 of copper sulfate and sodium carbonate at the same concentration, the best experimental result would gained when the primary product was kept for 24 hours under 75℃.

Teaching evaluation should be integrated with curriculum and teaching activities. Taking experiment for chemistry didactics as an example, the performance assessment form was made with evaluating a full process of "before class, during class and after class" after localization of performance assessment from abroad. According to the teaching mode of the same course in China, the form was used within the teaching process. A clear aim, concrete performing tasks, and explicit grading rules solved the problem of less teachers and more students, and met the requirement of high teaching quality and less lessons.

The students of grade 2012 and grade 2013 were selected as the experimental subjects, and the experimental group was treated with the model-assisted teaching, and the control group was conducted with the normal teaching. The result showed that the experimental group behaved better than the control group, which indicated that model-assisted teaching could improve preservice chemistry teachers' experimental demonstration skill significantly.

Based on the reform of trimester in University of Science and Technology of China (USTC), the public elective course of "The Popular Science Experiments of Chemistry in Life" was opened for the junior undergraduates who did not major in chemistry. The contents of the course were mainly related to the hot issues about life. "The determination of trace elements in hair" was one of the experimental projects, choosing students' hair as research object. And the contents and procedures of the experiment were also designed reasonably, which could stimulate students' interests in chemistry experiment, and further deepen the understanding of chemistry.

To improve the cultivating quality of undergraduate in the school of pharmacy, Ningxia medical university, a new organic and medicinal chemistry course group was carried out, and the problem-based learning (PBL) was applied to it. The discovery, design, synthesis and pharmacological evaluation of Conmana and the CFDA's approval process were integrated. PBL could help students to have a better understanding of pharmacy, arise the enthusiasm of students' desire for knowledge, enhance students' autonomous learning abilities, and train the cultivation of the team cooperation.

In order to meet the needs of discipline development and the cultivation of applied talents, this paper explored the medical chemistry design experiment of the synthesis of substrate and the determination of NAG enzyme for medical students.Experiment contents combined with clinical application of NAG enzyme of urine, substrate synthesis and detection method establishment, etc.It covered the basic theoretical knowledge and experimental skills of enzymology, organic chemistry, inorganic chemistry, analytical chemistry and other disciplines. The experiment was closely linked to the medical specialty, and combined theoretical knowledge with clinical examination. It could cultivate students' scientific literacy, thinking method, technical application ability and comprehensive ability.

Under the background of the transformation of higher education in China, the teaching practice of computer-aided design in reaction engineering provides an application example for the cultivation of applied talents. On the basis of the specific industrial cases in reaction engineering, a series of FORTRAN structured program for reaction kinetics and reactor design were prepared. Correspondingly, the syllabus and teaching plan were also adjusted. Then the course teaching in reaction engineering was carried out using computer-aided design, and typical simulation results were summarized. According to the classroom effects, an atmosphere of learner-centered teaching and learning forms, in which the theory and practice were combined together upon classroom teaching.

The structure of SO₃ was optimized and compared by the ab initio methods of MP2, QCISD, CCSD and density function theory (DFT) method of B3LYP under the basis set of 6-311++G(3df，3pd), and the results showed the optimization geometry parameters were optimal through QCISD/6-311++G(3df，3pd) method. Based on this method, the hybrid orbitals, hybrid modes and delocalization π bonds of SO₃ were analyzed through the natural bond orbital (NBO) theory. The results reveal that S adopts sp² equivalent hybridization and each sp² orbital is resided by one pair electrons and remaining an unfilled 3p_z orbital. O atoms adopt sp³ nonequivalent hybridization. In fact, the σ bonds between S and O atoms are S→O coordination bonds. The coupling of three 2p_z lone pair electrons in O atoms with empty 3p_z orbital of S atom forms the π⁶₄ delocalization π bond in SO₃ molecule. The Mayer bond order is 1.77 between S and O atoms, which further verifies the obvious double bond property of S=O bonds.