Brief Introduction of Non-Canonical Amino Acid Incorporation Technique for Introduction of Lysine Post-Translational Modifications into Proteins
WANG Zhipeng1,2, LI Man1,3, CHENG Nong-Yi4, WANG Tian5
1. Department of Chemistry, Texas A & M University, College Station, TX, USA 77840;
2. Department of Chemistry, Tsinghua University, Beijing 100084, China;
3. Department of Plant Pathology and Microbiology, Texas A & M University, College Station, TX, USA, 77840;
4. University of Wisconsin-Madison, Madison, WI, USA 53706;
5. Division of Biological Science, University of California San Diego, La Jolla, USA 92093
Abstract An increasing amount of protein lysine post-translational modifications (PTMs) have been gradually discovered recently. Besides their wide existence, these modifications play significant regulatory functions over chromatin structure and gene expression. However, it has been proved difficult to prepare sufficient amount of proteins with PTMs at specific positions, which makes the development of methodology to be an important sub-field for epigenetics. This paper will focus on the introduction of non-canonical amino acid incorporation technique and its application for the incorporation of lysine PTMs. We hope it can contribute to further chemistry major education and research in route.
WANG Zhipeng, LI Man, CHENG Nong-Yi, WANG Tian. Brief Introduction of Non-Canonical Amino Acid Incorporation Technique for Introduction of Lysine Post-Translational Modifications into Proteins[J]. Chinese Journal of Chemical Education, 2018, 39(18): 9-13.
