Design of Comprehensive Chemistry Experiment Based on Cultivation of Overall Quality Taking Determination of Tocopherols in Cereal Grains as Example
CAO Jiang-Ping1,2, DI Hong-Wei1,2, LIANG Yong-Feng1,2, LIU Shi-Wei1,2
1. College of Chemistry and Chemical Engineering, Ningxia Normal University, Guyuan 756000, China; 2. Engineering and Technology Research Center of Liupanshan Resources, Ningxia Normal University, Guyuan 756000, China
Abstract A comprehensive experiment has been designed named determination of tocopherols in cereal grains by using dispersive liquid-liquid microextraction (DLLME) coupled with high performance liquid chromatography(HPLC), and was applied to the chemical basic comprehensive experiment teaching. This experiment is designed on the basis of DLLME, a novel sample pretreatment technique. By consulting the relevant literature, the suitable extraction mode, dispersion strategy, extraction device, evaluating indicators of extraction effect, separation and analysis conditions of HPLC were determined. The design is based on recent scientific research, which can strengthen the operational skills of basic chemistry experiments, and arouse the students' enthusiasm in exploring the unknown and enhance the innovative ability and overall quality.
CAO Jiang-Ping, DI Hong-Wei, LIANG Yong-Feng, LIU Shi-Wei. Design of Comprehensive Chemistry Experiment Based on Cultivation of Overall Quality Taking Determination of Tocopherols in Cereal Grains as Example[J]. , 2019, 40(12): 53-55.
