Abstract In order to solve problems such as disconnection between professional content and English learning,unsatisfactory teaching effect in bilingual teaching of biochemistry, the bilingual teaching mode of biochemistry experiment course based on Content and Language Integrated Learning (CLIL) was carried out. On the basis of the traditional bilingual teaching mode, CLIL bilingual education of experiments provided effective application environment for professional English learning by integrating the language with professional knowledge in the form of specific laboratory teaching. By practicing CLIL bilingual education on experimental courses, the students can learn and apply professional English by setting up a real English scene. CLIL bilingual teaching has been improved on the traditional bilingual teaching method. It successfully stimulated learning enthusiasm and initiative of students by combining experimental content with professional English. CLIL-based bilingual teaching mode can achieve the goal of improving professional English level while learning subject knowledge.