Abstract Acetohydroxyacid synthase (AHAS) is the target enzyme of several types of super-efficient commercial herbicides attracting great international attention. The mutation of key residues of AHAS is the source of herbicide resistance formation. The nature and function of wild type and two single-site mutants of AHAS were studied thoroughly from gene cloning and mutation, protein expression and purification, to enzyme assay. Many basic manipulation and several challenging design experiments were included in the whole experiment process, in which the comprehensive theory and operation of chemical biology were involved. The course will help the students in principle to understand the necessity of pesticide application, the cause of pesticide resistance, design and development of new green pesticides from the perspective of molecular structure.
OU-YANG Di, NIU Cong-Wei, XI Zhen. Application of Study of Pesticide Target Resistance in University Comprehensive Experiment of Chemical Biology[J]. Chinese Journal of Chemical Education, 2021, 42(18): 113-120.
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WANG Heng, WU Xiao-Chun. Deciphering Glyphosate[J]. Chinese Journal of Chemical Education, 2019, 40(18): 12-16.