Abstract Based on the practical results of students in enterprises, the comprehensive innovative experiment was explored and optimized based on the quality control of unknown chemical impurities in amino acid bulk drug. Students were encouraged to detect unknown chemical impurities in L-isoleucine by gas chromatography-mass spectrometry combined with headspace solid phase microextraction (HS-SPME-GC-MS). The qualitative and quantitative chromatographic methods were developed to determine impurity by using 2-methylbutyraldehyde as standard substance. The results show that none exceeded the national limit standard. Though the experiment,students could not only master the basic theory and method of quality control in analytical chemistry, obtain the experimental principle and operation of HS-SPME-GC-MS, understand green chemical pretreatment methods, be familiar with qualitative and data analysis software, but also stir up their subjective interests, improve the ability to comprehensively analyze and solve practical problems, and enhance the ability of scientific research innovation and teamwork.
LIU Lei, GAO Yue-Ying, TANG Jia-Yue, LI Hui, HUI Ren-Jie. Design and Exploration of Comprehensive Innovative Experiment of Analytical Chemistry Based on Unknown Chemical Impurities Detection in Amino Acids[J]. Chinese Journal of Chemical Education, 2022, 43(20): 85-91.
