Abstract This paper explores the practical pathways and implementation outcomes of innovatively introducing circular dichroism(CD) spectroscopy,an advanced analytical technique,into undergraduate experimental teaching.Centered on amino acid chirality recognition and nucleic acid G-quadruplex conformation analysis,a systematic experimental teaching program based on CD spectroscopy was developed,thereby extending its application within undergraduate education.The curriculum begins with the CD response of amino acid enantiomers as a foundational concept,guiding students to understand the principles of chiral recognition before advancing to a progressive exploration of conformational changes of human telomeric DNA and VEGF promoter region G-quadruplexes induced by metal ions such as K+ and Na+.Through real-time spectral monitoring,the molecular mechanisms of G-quadruplexes in gene regulation and anti-tumor drug research are demonstrated.Teaching practice shows that this course not only effectively enhances students’ experimental skills and scientific thinking but also,through the integration of cutting-edge techniques and molecular biology,stimulates their interest in bioanalytical technologies,which fully demonstrates the innovative potential and application value of CD spectroscopy in undergraduate experimental teaching.
ZHAO Ling-Li, MAO Zhen-Jun, CHE Hai-Yan, SHEN Hong. Implementing Circular Dichroism in Experimental Teaching:From Amino Acid Chirality to Nucleic Acid G-Quadruplex Conformation[J]. Chinese Journal of Chemical Education, 2026, 47(18): 47-54.
