1. College of Science, China University of Petroleum, Beijing 102249, China;
2. College of Chemical Engineering, China University of Petroleum, Beijing 102249, China
Abstract The type of adsorbent is one of the most important factors in column chromatography separation. Several common stationary phases have been adopted to analyze their influence on the separation effect of fluorescent yellow and methylene blue BB, such as silica gel, alkaline alumina, neutral alumina, and acidic alumina, etc. The acid distribution and pore distribution of the adsorbent were characterized by NH3-TPD and N2 adsorption desorption respectively. The molecular size of fluorescent yellow and methylene blue was calculated using density functional method. The relationship between the acidity, the structure of the adsorbent and the separation efficiency was also investigated. Experimental results showed that the separation effect of silica gel was better than that of neutral alumina. In addition, fluorescent yellow was eluted firstly when silica gel was used as adsorbent, which was contrary to results when neutral alumina was employed. This process was helpful for students to clearly understand the function of stationary phase and column chromatography. And scientific research could also make sense for the cultivation of earnest scientific attitude and ability to analyze and solve problems.
GUO Qiao-Xia, ZHOU Jing, YUAN Gui-Mei, YU Xin, ZHAO Liang, ZHANG Min, LIU Zhi-Chang, SHEN Bao-Jian. Effect of Adsorbent on Column Chromatography to Separate Fluorescent Yellow and Methylene Blue BB[J]. Chinese Journal of Chemical Education, 2017, 38(10): 47-52.
