Application of Semimicroscale and Microscale Technique in the Extraction and Separation of Spinachrome
LI Zhi-Wei1, KANG Shao-Ying2, LI Jiang-Sheng1, ZHANG Ling1
1. School of Chemistry and Food Engineering, Changsha University of Science&Technology, Changsha 410114, China; 2. Hunan Institute of Food Quality Supervision Inspection and Research, Changsha 410111, China
Abstract The extraction and separation of spinachrome constitutes a very important section in organic chemistry experiment of chemistry and chemical engineering specialty etc. But there exist some problems such as taking long experimental time, using too much solvent and making serious environmental pollution. In this paper, a series of factors including the spinach dosage, extraction solvent amount, stationary phases, column chromatography size, elution solvents and their dosage were explored using the semimicroscale and microscale technique. It has been found that the main pigments in spinach were well separated in 29 min over 4 grams of silica gel as the stationary phase using 51 mL of petroleum ether and ethyl acetate (3:2) as the elute, after sequential extraction with 5 mL of methanol, and 5 mL of the mixture of petroleum ether and methanol (3:2), respectively, in a scale of 5 grams of spinach leaves as the staring material.
LI Zhi-Wei, KANG Shao-Ying, LI Jiang-Sheng, ZHANG Ling. Application of Semimicroscale and Microscale Technique in the Extraction and Separation of Spinachrome[J]. , 2019, 40(24): 55-57.
