Discussion on the Relative Molecular Weight Estimation for Biomacromolecules
WANG Zhipeng A1,2,3*, JIANG Zhen-Xiong4,5, CHE Zi-Liang3, MA Xin-Yu2, WANG Lu3, JIANG Han-Jie1, WANG Peng6, ZHENG Shao-Yong7
1. Division of Genetics, Department of Medicine, Brigham and Women's Hospital; Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA;
2. Department of Chemistry, Texas A&M University, College Station, TX 77840, USA;
3. Department of Chemistry, Tsinghua University, Beijing 100084, China;
4. Department of Plant and Pathology and Microbiology, Texas A&M University, College Station, TX 77840, USA;
5. Department of Biology, Texas A&M University, College Station, TX 77840, USA;
6. Burn Surgery, The First Affiliated Hostital, Sun Yat-sen University, Guangzhou 510080, China;
7. Department of Food Science, Jinshan College of Fujian Agriculture and Forestry University, Fujian 350000, China
Abstract Relativemolecularweight calculation plays a nonnegligible role in the scientific quantitative analysis. However, not all calculations can be made with high accuracy: under certain circumstances the length-based estimation is the only way forrelative molecular weight estimation. Thispapersystemically introduces a feasible method based on the averagedrelative molecular weight of monomers. Single-stranded and double-stranded deoxyribonucleic acid, ribonucleic acid, and proteins are selected here for analysis in order to elucidate and compare the maximal biases based on this methodology. Meanwhile, one rarely explored tip about double stranded DNA is mentioned.
WANG Zhipeng A, JIANG Zhen-Xiong, CHE Zi-Liang, MA Xin-Yu, WANG Lu, JIANG Han-Jie, WANG Peng, ZHENG Shao-Yong. Discussion on the Relative Molecular Weight Estimation for Biomacromolecules[J]. Chinese Journal of Chemical Education, 2020, 41(12): 91-98.
