Abstract In this paper, a new method for the determination of catalase activity by dead stop titration was established. In hydrochloric acid medium, the quantitative reaction of ceric ammonium sulfate (Ce4+) with H2O2 is 2∶1. When H2O2 was titrated with Ce4+ standard solution, the activity of catalase was determined by the change of H2O2 before and after enzyme decomposition. The results showed that there was a linear relationship between enzyme activity X and Ce4+standard solution, and the titration volume difference in the range of 0.000~141U ΔV(Ce4+)=0.201 0X-0.067 9. Meanwhile, the correlation coefficient was 0.999 4 and the check out the quantity was 0.400 U. The method was applied to the determination of catalase activity in living tissues of grass carp.
TAO Suo-Jun, ZHANG Ai-Jv, ZHANG Xiao-Lin. Design on Experiment of Determination of Catalase Activity by Dead Stop Titration[J]. Chinese Journal of Chemical Education, 2023, 44(20): 111-114.
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LIAO Yan-Yan, MENG Jiao-Long, JIANG Xue-Feng. Peptide Chemistry in Cosmetics[J]. Chinese Journal of Chemical Education, 2022, 43(22): 1-11.
