Cross-Disciplinary Teaching Design Based on Cognitive Model Construction in Chemistry and Biology: Investigating the Kinetics of Sucrase-Catalyzed Sucrose Hydrolysis Using Commercial Glucometer
Abstract Quantitative study of sucrose hydrolysis has been difficult to carry out in depth at the high school level due to the limitations of traditional detection methods. This study introduces a commercial blood glucose meter as the detection tool and takes the hydrolysis of sucrose catalyzed by yeast invertase as the research subject,integrating knowledge from chemical kinetics and biological enzymology to design an interdisciplinary experimental teaching program that can be completed within a relatively short time. Students collect data based on the specific recognition principle of glucose oxidase and conduct parallel experiments at three temperatures (25,30,35 ℃),sequentially constructing a glucose concentration-time variation model,an initial reaction rate calculation model,and an Arrhenius activation energy determination model,ultimately obtaining the activation energy value for the enzyme-catalyzed hydrolysis. This process helps students deepen their understanding of concepts such as reaction rate and activation energy,gives them firsthand appreciation of the critical role of enzyme specificity in experimental detection,and develops their abilities in function fitting and mathematical modeling through the data processing stage. Centered on the progressive construction of cognitive models,this design provides a practical and operable pathway for fostering students’ core disciplinary competencies.
SHANG Hao, ZHOU Yan. Cross-Disciplinary Teaching Design Based on Cognitive Model Construction in Chemistry and Biology: Investigating the Kinetics of Sucrase-Catalyzed Sucrose Hydrolysis Using Commercial Glucometer[J]. Chinese Journal of Chemical Education, 2026, 47(15): 46-53.
