Experimental Design for Simulating Antioxidant Activity Based on the Redox Reaction Between Ferric Chloride and Vitamin C
LI Jing-Yao1, HUANG Bao-Sheng2*
1. Competitive Program Department,Class 2,Grade 10,Beizhen High School of Shandong Province,Binzhou 256600, China; 2. School of Biological and Pharmaceutical Engineering,Shandong University of Aeronautics,Binzhou 256600, China
Abstract Based on the redox reaction between ferric chloride and vitamin C, integrating the basic biological principle that “antioxidants scavenge reactive oxygen species (ROS)”, this study simulates and investigates the antioxidant activity of vitamin C in vitro. Experimental results show that: with the increase in the molar concentration of vitamin C in the solution, the degree of reduction of Fe3+ (tan) to Fe2+ (pale green) is enhanced; the pH of the solution decreases from 2.3 in group 0# to 0.9 in group 4#; through the KSCN colorimetric test, the solution gradually changes from blood-red in group 0# to colorless in group 4#. These findings confirm that vitamin C possesses in vitro antioxidant activity. The study aligns with the core competencies of senior high school chemistry, such as “macroscopic identification and microscopic analysis” and “evidence-based reasoning and model cognition”, providing a practical case for interdisciplinary learning and the implementation of the assessment orientation of the “new college entrance examination”.
LI Jing-Yao, HUANG Bao-Sheng. Experimental Design for Simulating Antioxidant Activity Based on the Redox Reaction Between Ferric Chloride and Vitamin C[J]. Chinese Journal of Chemical Education, 2026, 47(7): 96-101.
