Abstract To solve the lack of experiment classes, which are quantitative analysis of dye solutions and determination of complex systems by spectrophotometry, the simultaneous determination of dye components (reactive red 195, reactive yellow 145 and reactive blue 19) by first derivative spectrophotometry was designed as an instrumental analysis experiment course. 655 nm, 566 nm and 326 nm were respectively selected as detection wavelengths for reactive blue 19, reactive red 195 and reactive yellow 145, and the pH value of the solution is adjusted between 4 and 7. The methods had great linear relationships with the first derivative spectra in the concentration range of 10~100 mg/L. Many groups of mixed dye solution were determined, the comparison between measured and actual values verified the precision and accuracy of the method. The experiment deepened the students’ understanding and applying of spectroscopy theoretical knowledge, expanded their vision in textile dyeing and printing analysis field, enhanced the ability to quantitatively analyze the multi-components.
LIU Hui-Qing, XU Zhong-Qi. Experimental Teaching Design for the Concentration Determination of Mixed Dyes by First Derivative Spectrophotometry[J]. Chinese Journal of Chemical Education, 2024, 45(18): 50-55.
