化学·生活·社会
基质辅助激光解析/电离飞行时间质谱(MALDI-TOF MS)在微生物鉴定中的应用*
闫雯倩1 , 陈企发1,2 , 夏炎1,2**
1.南开大学化学学院分析科学研究中心 天津市生物传感与分子识别重点实验室 天津 300071;
Application of Matrix Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry (MALDI-TOF MS) in Microbial Identification
YAN Wen-Qian1 , CHEN Qi-Fa1,2 , XIA Yan1,2**
1. Tianjin Key Laboratory of Biosensing and Molecular Recognition, Research Center for Analytical Science, College of Chemistry, Nankai University, Tianjin 300071, China;
摘要： 基质辅助激光解析/电离飞行时间质谱(MALDI-TOF MS)是一种新型软电离生物质谱,具有检测速度快、操作简便、结果准确等优点,目前已成为可靠的微生物快速鉴定技术。就工作流程而言,与常规生化方法相比,MALDI-TOF MS可以将微生物鉴定的时间缩短为一天甚至更短。对于具有抗生素耐药性的微生物,使用MALDI-TOF MS鉴定也有很好的准确性。在病毒鉴定中,MALDI-TOF MS也可以发挥作用,已有报道将MALDI-TOF MS和机器学习(ML)分析方法结合来检测鼻拭子中SARS-CoV-2。此外,MALDI-TOF MS还可用于细菌的无光谱库鉴定。目前,MALDI-TOF MS正通过与其他技术(例如傅里叶红外光谱FTIR)相结合进一步扩大微生物鉴定范围。
关键词： 基质辅助激光解析/电离飞行时间质谱(MALDI-TOF MS) ,
微生物鉴定 ,
抗生素耐药性 ,
新冠病毒检测
基金资助: *国家自然科学基金资助项目(21874073);南开大学沧州渤海新区绿色化工研究院基金项目(NCC2020PY11)
通讯作者:
**E-mail: nkxiayan@nankai.edu.cn
引用本文:
闫雯倩, 陈企发, 夏炎. 基质辅助激光解析/电离飞行时间质谱(MALDI-TOF MS)在微生物鉴定中的应用* [J]. 化学教育（中英文）, 2022, 43(10): 7-14
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