|
化学·生活·社会
|
|
|
|
|
|
| 激光扫描共聚焦显微镜的应用与发展趋势 |
| 潘庆芝, 李春光* |
| 吉林大学化学学院无机合成与制备化学全国重点实验室 吉林长春 130012 |
|
| Applications and Developments of Confocal Laser Scanning Microscope |
| PAN Qing-Zhi, LI Chun-Guang* |
| State Key Laboratory of Inorganic Synthesis and Preparative Chemistry,College of Chemistry, Jilin University,Changchun 130012,China |
|
|
摘要:介绍了光学显微镜的重要代表——激光扫描共聚焦显微镜(CLSM)。通过采用激光光源、共焦光学系统和数字图像处理技术,CLSM可以在较短时间内获得高对比度、高分辨率的图像,减少样品受光损害的风险。CLSM广泛应用于细胞生物学、医学、药物开发和材料科学等领域,为科学研究提供了重要的支撑。通过深入了解CLSM的原理及应用,研究人员可以更有效地设计实验和分析数据,获得更精确的研究结果。探讨了CLSM的基本原理、应用领域及其未来发展趋势,强调其在促进高水平科学研究中的潜力和价值。
|
|
| 关键词: 共聚焦显微镜,
细胞结构,
荧光标记,
材料科学,
纳米材料
|
|
收稿日期: 2024-09-06
|
|
通讯作者:
*E-mail:cglee@jlu.edu.cn
|
| 引用本文: |
|
潘庆芝, 李春光. 激光扫描共聚焦显微镜的应用与发展趋势[J]. 化学教育(中英文), 2025, 46(12): 1-6
|
|
| [1] |
Mehta D S.Proceedings of the National Academy of Sciences,India Section A:Physical Sciences,2018,88:437-451
|
| [2] |
李川,王磊.化学教育(中英文),2018,39(12):1-10
|
| [3] |
Bucur O,Zhao Y.Frontiers in Medicine,2018,5:322
|
| [4] |
Sheppard C J R.Applied Sciences,2021,11(19):8981
|
| [5] |
Sevick-Muraca E M.Nature Nanotechnology,2022,17:568-569
|
| [6] |
Beil M,Irinopoulou T,Vassy J,et al.Journal of Microscopy,1996,183(3):231-240
|
| [7] |
Menéndez B,David C,Nistal A M.Computers & Geosciences,2001,27(9):1101-1109
|
| [8] |
Jonkman J,Claire M B,Graham D W,et al.Nature Protocols,2020,15:1585-1611
|
| [9] |
Guida S,Arginelli F,Farnetani F,et al.Applied Sciences,2021,11(5):1979
|
| [10] |
张薇,郭玉鹏,张文科.化学教育(中英文),2022,43(14):57-60
|
| [11] |
Bailey G W,Jerome W G,Mckernan S,et al.Microscopy and Microanalysis,1999,5:988-989
|
| [12] |
Schüürmann M,Stecher M M,Paasch U,et al.Journal of the European Academy of Dermatology and Venereology,2020,34(7):1496-1499
|
| [13] |
Olivo M,Lau W,Manivasager V,et al.International Journal of Oncology,2003,23(4):983-990
|
| [14] |
Ramírez M J,Surrallés J.Cytogenetic and Genome Research,2008,122(3):237-242
|
| [15] |
Villard A,Breuskini I,Casiraghi O,et al.Oral Oncology,2022,127:105826
|
| [16] |
Yang Y,Wang N,Wang Z,et al.Chemical Engineering Journal,2023,475:145764
|
| [17] |
Liu Y,Zhao J,Yang X,et al.Anti-Corrosion Methods and Materials,2022,69(6):592-602
|
| [18] |
Kilmametov A,Gröger R,Hahn H,et al.Advanced Materials Technologies,2017,2(1):1600115
|
| [19] |
Tang C,Bao T,Li S,et al.Applied Catalysis B:Environment and Energy,2024,342:123384
|
| [20] |
He Y,Chen C,Liu Y,et al.Nano Letters,2022,22(12):4970-4978
|
| [21] |
刘欢,刘克文.化学教育,2014,35(24):1-6
|
|
|
|