Abstract The calculation of titration error is an important content in analysis chemistry. In most textbooks, there are too multifarious calculation formulas of titration error to be grasped for students. To address the problem, we redefined titration error by using the volume ratio between titrant solution and analyte solution. Further more, general calculation formula of acid-base titration error was constructed according to proton balancing principle. It would be achievable to precisely calculate titration error in various acid-base titrations by applying the above formula.