WANG Zhipeng A1,2,3*, JIANG Zhen-Xiong4, CHE Zi-Liang5,6, MA Xin-Yu2, WANG Peng7
1. Division of Genetics,Department of Medicine,Brigham and Women’s Hospital; Department of Biological Chemistry and Molecular Pharmacology,Harvard Medical School,Boston,MA,02115,USA;
2. Department of Chemistry,Texas A&M University,College Station,TX,77840,USA;
3. Department of Chemistry,Tsinghua University,Beijing 100084,China;
4. Department of Biology,Texas A&M University,College Station,TX,77840,USA;
5. Department of Mathematics, Harvard University; MA 02138,USA;
6. Citadel Securities,LLC; 131 South Dearborn St. Chicago Illinois 60603,USA;
7. Department of Burn Surgery,First Affiliated Hospital of Sun Yat-Sen University,Guangzhou 510080,China
Abstract Gel electrophoresis is a common and important techniques used in protein biochemistry,biophysics,and chemical biology research. However,the knowledge about protein structure and function behind it has been so long ignored by undergraduate education. Starting from the criteria filtering proper molecular-weight size marker for protein electrophoresis,this paper analyzed and contrasted several classes of molecular-weight size marker,and offered some reasonable suggestions for selecting optimal protein marker based on the stability analysis of primary structure and advanced structure.
WANG Zhipeng A, JIANG Zhen-Xiong, CHE Zi-Liang, MA Xin-Yu, WANG Peng. Discussion on Protein Molecular Weight Markers[J]. Chinese Journal of Chemical Education, 2022, 43(6): 105-110.
