Comprehensive Chemical Biology Experiment Combining NUPACK Computationally Aided Design of Chain Displacement Amplification Strategies with Gel Electrophoresis Validation
LUO Lan, XING Yu-Kun, LIU Ji-Xiang, HE Yuan, OU-YANG Xiang-Yuan**, CHEN Fang-Fang**
College of Chemistry and Materials Science,Northwest University,Xi'an 710127,China
Abstract This study introduces an integrated experimental approach employing strand displacement amplification(SDA)technology for the sensitive detection of Mucin 1 glycoprotein.The stability and hybridization efficiency of the designed DNA hairpin structures were predicted using the NUPACK software,and the feasibility of the SDA process was validated through polyacrylamide gel electrophoresis.This educational approach,integrating computational simulations with experimental procedures,provides a comprehensive learning platform for students.It facilitates a deep understanding of nucleic acid structural stability,hybridization efficiency,and amplification mechanisms.The methodology is designed to stimulate students’ interests in learning and significantly contributes to the development of their experimental skills and scientific reasoning,preparing them for future challenges in scientific research.
LUO Lan, XING Yu-Kun, LIU Ji-Xiang, HE Yuan, OU-YANG Xiang-Yuan, CHEN Fang-Fang. Comprehensive Chemical Biology Experiment Combining NUPACK Computationally Aided Design of Chain Displacement Amplification Strategies with Gel Electrophoresis Validation[J]. Chinese Journal of Chemical Education, 2025, 46(10): 55-62.
